Journal: eLife

Article Title: DCC regulates astroglial development essential for telencephalic morphogenesis and corpus callosum formation

doi: 10.7554/eLife.61769

Figure Lengend Snippet:

Article Snippet: Primary antibodies used for immunohistochemistry were rabbit anti-APC (1:250, ab15270, Abcam), mouse anti-α-dystroglycan (1:250, clone IIH6C4, 05-593, Merck), rabbit anti-β-catenin (1:500, 9562, Cell Signaling Technology), mouse anti-β-dystroglycan (1:50, MANDAG2, 7D11, Developmental Studies Hybridoma Bank), chicken anti β-galactosidase (1:500, ab9361, Abcam), rabbit anti-cleaved-caspase 3 (1:500, 9661, Cell Signaling Technology), goat anti-DCC (1:500, sc-6535, Santa Cruz Biotechnology), mouse anti-Gap43 (1:500; MAB347, Millipore), mouse anti-Gfap (1:500; MAB3402, Millipore), rabbit anti-Gfap (1:500; Z0334, Dako), mouse anti-Glast (or Eaat1; 1:500; ab49643, Abcam), rabbit anti-Glast (or Eaat1; 1:250; ab416, Abcam), mouse anti-Ki67 (1:500; 550609, BD Pharmingen), chicken anti-Laminin (1:500; LS-C96142, LSBio), rabbit anti-Laminin (1:500; L9393, Sigma), mouse anti-N-cadherin (CDH2; 1:250, 610921, BD Biosciences), rat anti-Nestin (NES; 1:50, AB 2235915, DSHB), chicken anti-Nestin (1:1000, ab134017, Abcam), goat anti-NTN1 (1:500; AF1109, R&D Systems), mouse anti-neurofilament (1:500; MAB1621, Millipore), rabbit anti-Nfia (1:500; ARP32714, Aviva Systems Biology), rabbit anti-Nfib (1:500; HPA003956, Sigma), rabbit anti-neuronal-specific-ßIII-tubulin (1:500; ab18207, Abcam), rabbit anti-phospho p44/42 Mapk (or Erk1/2; 1:250; 9101, Cell Signaling Technology), rabbit anti-SOX9 (1:500, AB5535, Merck) and goat anti-TDTOMATO (1:500, ab8181-200, Sicgen).

Techniques: Droplet Countercurrent Chromatography, Western Blot, Immunofluorescence, Recombinant, shRNA, CRISPR, In Situ Hybridization, Sequencing, Mutagenesis, Generated, Imaging, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis

doi: 10.3892/etm.2017.4632

Figure Lengend Snippet: Expression of KLF8 was detected in 8 paired cases of lung adenocarcinoma and adjacent non-tumor tissue. GAPDH was used as a loading control. T, tumor tissue; N, non-tumor tissue; KLF8, Kruppel-like factor 8.

Article Snippet: Then the samples were washed with phosphate-buffered saline with Triton X-100 three times, and incubated with rabbit anti-human KLF8 antibody (1:150; Aviva Systems Biology Corp.) or rabbit anti-human Ki67 (1:500; ab15580; Abcam, Cambridge, UK) overnight at 4°C.

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis

doi: 10.3892/etm.2017.4632

Figure Lengend Snippet: Expression of (A-D) Ki67 and (E-H) KLF8 in lung adenocarcinoma and adjacent non-tumor tissue by immunohistochemistry. (A and E) Adjacent non-tumor tissue. (B and F) Well-differentiated LAC. (C and G) Moderately differentiated LAC. (D and H) Poorly differentiated LAC. Magnification, ×200; Scale bar, 50 µm. KLF8, Kruppel-like factor 8, LAC, lung adenocarcinoma.

Article Snippet: Then the samples were washed with phosphate-buffered saline with Triton X-100 three times, and incubated with rabbit anti-human KLF8 antibody (1:150; Aviva Systems Biology Corp.) or rabbit anti-human Ki67 (1:500; ab15580; Abcam, Cambridge, UK) overnight at 4°C.

Techniques: Expressing, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis

doi: 10.3892/etm.2017.4632

Figure Lengend Snippet: KFL8 and Ki67 expression and clinicopathological parameters in 140 lung adenocarcinoma samples.

Article Snippet: Then the samples were washed with phosphate-buffered saline with Triton X-100 three times, and incubated with rabbit anti-human KLF8 antibody (1:150; Aviva Systems Biology Corp.) or rabbit anti-human Ki67 (1:500; ab15580; Abcam, Cambridge, UK) overnight at 4°C.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis

doi: 10.3892/etm.2017.4632

Figure Lengend Snippet: Correlation between expression of KLF8 and Ki67 in 140 lung adenocarcinoma specimens.

Article Snippet: Then the samples were washed with phosphate-buffered saline with Triton X-100 three times, and incubated with rabbit anti-human KLF8 antibody (1:150; Aviva Systems Biology Corp.) or rabbit anti-human Ki67 (1:500; ab15580; Abcam, Cambridge, UK) overnight at 4°C.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis

doi: 10.3892/etm.2017.4632

Figure Lengend Snippet: Correlation between KLF8 and Ki67 expression in lung adenocarcinoma was further evaluated by Pearson's rank correlation. KLF8, Kruppel-like factor 8.

Article Snippet: Then the samples were washed with phosphate-buffered saline with Triton X-100 three times, and incubated with rabbit anti-human KLF8 antibody (1:150; Aviva Systems Biology Corp.) or rabbit anti-human Ki67 (1:500; ab15580; Abcam, Cambridge, UK) overnight at 4°C.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis

doi: 10.3892/etm.2017.4632

Figure Lengend Snippet: Overall survival curve of patients with lung adenocarcinoma according to high or low expression of (A) KLF8 and (B) Ki67. KLF8, Kruppel-like factor 8.

Article Snippet: Then the samples were washed with phosphate-buffered saline with Triton X-100 three times, and incubated with rabbit anti-human KLF8 antibody (1:150; Aviva Systems Biology Corp.) or rabbit anti-human Ki67 (1:500; ab15580; Abcam, Cambridge, UK) overnight at 4°C.

Techniques: Expressing

Journal: Hepatology (Baltimore, Md.)

Article Title: Etiology-dependent molecular mechanisms in human hepatocarcinogenesis.

doi: 10.1002/hep.22033

Figure Lengend Snippet: Fig. 3. Overexpression of MDM4 and EEF1A2 in HCC. (A) MDM4 and EEF1A2 gene expression in HCC measured by QRT-PCR. Expression data are log2 transformed and plotted as ratios relative to expression in normal liver. (B) Exemplary western blot analyses of MDM4 and EEF1A2 proteins in primary HCCs (3 of each etiologic group analyzed) and normal liver.

Article Snippet: Immobilized proteins were incubated with primary antibodies [monoclonal mouse anti-human beta-actin, 1:15,000 (MP Biomedicals, Heidelberg, Germany); monoclonal mouse anti-human EEF1A2, 1:5000 (Upstate Biotechnology Inc., Waltham, MA); polyclonal rabbit anti-human MDM4, 1:8000 (Aviva Systems Biology, San Diego, CA)] and horseradish peroxidase–linked anti-mouse or rabbit secondary antibodies (1:2000) (Cell Signaling Technology, Beverly, MA).

Techniques: Over Expression, Gene Expression, Quantitative RT-PCR, Expressing, Transformation Assay, Western Blot

Journal: Hepatology (Baltimore, Md.)

Article Title: Etiology-dependent molecular mechanisms in human hepatocarcinogenesis.

doi: 10.1002/hep.22033

Figure Lengend Snippet: Fig. 5. Knockdown experiments of MDM4 and EEF1A2 in HepG2 and Hep3B cell lines. (A) BrdU prolifer- ation assay with gene-specific and control siRNAs. Inhibition of MDM4 and EEF1A2 expression decreased HepG2 and Hep3B proliferation rates. (B) MTT cell viability assay using gene-specific and control siRNA. Inhibition of MDM4 and EEF1A2 expression decreased HepG2 and Hep3B cell viability. (C) PI-FACS analysis of cells transfected with gene-specific or control siRNAs. Inhibition of MDM4 and EEF1A2 ex- pression increased HepG2 and Hep3B apoptosis rates. Asterisks in- dicate significant (P 0.01) differ- ences compared with untreated cells.

Article Snippet: Immobilized proteins were incubated with primary antibodies [monoclonal mouse anti-human beta-actin, 1:15,000 (MP Biomedicals, Heidelberg, Germany); monoclonal mouse anti-human EEF1A2, 1:5000 (Upstate Biotechnology Inc., Waltham, MA); polyclonal rabbit anti-human MDM4, 1:8000 (Aviva Systems Biology, San Diego, CA)] and horseradish peroxidase–linked anti-mouse or rabbit secondary antibodies (1:2000) (Cell Signaling Technology, Beverly, MA).

Techniques: Knockdown, Control, Inhibition, Expressing, Viability Assay, Transfection

Journal: Frontiers in Molecular Biosciences

Article Title: Impact of Pals1 on Expression and Localization of Transporters Belonging to the Solute Carrier Family

doi: 10.3389/fmolb.2022.792829

Figure Lengend Snippet: SLC genes are strongly downregulated in Pals1 haploinsufficient kidneys. More than 100 genes of the SLC superfamily ( Pizzagalli et al., 2021 ) are downregulated in Pals1-haploinsufficient kidneys (see Supplementary Material 9 ; Weide et al., 2017 ). The table shows ten SLC genes i ) that are known to be highly expressed in the kidney, ii ) functionally and phylogenetically conserved in mammalian species (mouse, dog, human) and iii ) among the most regulated genes identified in the transcriptome of Pals1-deficient kidneys.

Article Snippet: Polyclonal antibodies (pAB) were used against Slc34a3 (Aviva Systems Biology Corporation, ARP32173 P050, 1:500), Slc5a2 (Novus Biologicals, NBP1-92384, 1:500) and Slc16a14 (Sigma-Aldrich, HPA040518, 1:500).

Techniques: Membrane